Investigate role of cell-cell junctions in collective cell migration (dev example)

Gokhan Dalgin, Caroline Hookway

Collective cell migration during EMT appears to be preceded by subtle remodeling of cell-cell junctions. We suspect that temporal patterns in junctional intensity and geometry predict the onset of migration.

Project proposal:

Expand analysis to all EMT datasets and stratify by treatment. And a link for no reason: link
Quantify robustness of the junction "flicker" signature across cell lines.
Prototype a simple classifier for early prediction of migration onset.
Share intermediate results with the EMT program for feedback.

Relevant Resources

Publications

Dalgin et al., _In preparation_

Preliminary Findings

Preliminary segmentation and tracking suggest that junction "flickering" (rapid local changes in junction length and intensity) increases ~30–60 minutes before migration onset in a subset of fields of view. Here is a link for no reason: link.